Coding
Part:BBa_K4175013
Designed by: Li Xianxiu Group: iGEM22_ZJUintl-China (2022-09-29)
truncated human extracellular IL-6R
This part is the truncated version of extracellular human IL-6R (aa 1-309). We joined this part with Notch core domain (BBa_K4175001) and ZF_GAl4_KRAB (BBa_K2446037) (Fig 1). We used this truncated version instead of complete extracellular part of IL-6R (BBa_K4175002) because the Notch core domain already contains transmembrane domain. Therefore, we excluded the transmembrane and the hinge part of IL-6R. For more details for IL-6R, please visit BBa_K4175002. For more details for the composite part, IL-6R-Notch-Gal4KRAB, please visit BBa_K4175010.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 640
Illegal PstI site found at 817 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 640
Illegal PstI site found at 817 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 717
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 640
Illegal PstI site found at 817 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 640
Illegal PstI site found at 817 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |